ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (2): 404-410.doi: 10.11843/j.issn.0366-6964.2016.02.026

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Prophylactic Effect of Compound Chinese Herbal Medicine on Mycoplasma suis Infection

DONG Yao-ze,LI Xiao-yun,ZHANG Jin-qiang,SHI Ze-yu,CHEN Jian-shan,LI Hong-quan,MA Hai-li*   

  1. (College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
  • Received:2015-07-13 Online:2016-02-23 Published:2016-02-23

Abstract:

The study aimed to develop compound Chinese herbal medicine that is more efficient in prevention and control of the M.suis infection.M.suis of positive blood were cultured in cell culture fluid with the compound Chinese herbal medicine Ⅰ,Ⅱ,Ⅲ and deoxytetracycline.The copies of M.suis in cell culture fluid were measured by qPCR every 12 h.Fifty clinical healthy,35 day-old piglets with natural infection of M.suis confirmed by PCR were randomly allocated to 5 groups,each with 10 replicates,and all the groups were fed separately.Piglets in three compound Chinese herbal medicine group were fed with a diet that contained 20 g•kg-1 of the compound Chinese herbal medicine Ⅰ,Ⅱ,and Ⅲ respectively for seven days;piglets in deoxytetracycline group were fed with a diet that mixed 200 mg•kg-1 of deoxytetracycline for five days;piglets in control group were fed with diet.Blood samples of piglets were collected by the anterior vena before (0 day) and after the usage of medicine at the 10th,20th and 30th days.The copies of M.suis in blood,erythrocyte superoxide dismutase,erythrocyte membrane ATPase (Na+K+-ATPase,Ca2+Mg2+-ATPase),lymphocyte proliferation rate (T,B),and the serum content of IgG were measured.The results showed that:the three compound Chinese herbal medicine could significantly decrease the copies of M.suis in vitro (P<0.01);compared with the deoxytetracycline group and the control group,the compound Chinese herbal medicine Ⅰ could significantly decrease the copies of M.suis in blood of piglets (P<0.01),improve the activity of erythrocyte superoxide dismutase and B-proliferation rate (P<0.05);the compound Chinese herbal medicine Ⅱ had positive effects on erythrocyte superoxide dismutase,the activity of erythrocyte membrane Na+K+-ATPase and lymphocyte proliferation rate (P<0.05),whereas it could not effectively reduce the M.suis copies;the compound Chinese medicine Ⅲ could reduce the M.suis copies in blood of piglets (P<0.01) and only improve the activity of erythrocyte superoxide dismutase (P<0.05).In conclusion,the compound Chinese medicineⅠhas favorable prophylactic effects on the M.suis infection.

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